P-13: Evaluation of Sperm Acrosin Activity and Chromatin Structure in Varicocele Patients Before and after Varicocelectomy
Authors
Abstract:
Background: Evaluation and comparison of total acrosin activity along with DNA integrity in the semen samples obtained from the varicocele individuals before and after the surgery and also in fertile individuals. Materials and Methods: Semen parameters, protamine deficiency, DNA fragmentation and Total acrosin activity were measured in patients with a clinical diagnosed varicocele of grade II or III varicocele men (n= 70) and fertile men as control group(n= 30). Protamine deficiency, DNA fragmentation and semen parameters were assessed by chromomycin A3, TUNEL staining and WHO criteria respectively. Total acrosin activity assessed by spectrophotometery. Results: Total acrosin activity, percentage CMA3 positive, TUNEL positive sperm and abnormal morphology are higher in the varicocele individuals before the surgery compared to the fertile individuals, yet all the mentioned criteria decreased significantly after the surgery. Conclusion: The results of this study reveal that varicocelectomy improved sperm concentration, morphology, DNA Fragmentation and protamine deficiency in varicocele patients. However, Total acrosin activity was higher in these individuals and decreased to the value close to fertile control post surgery.
similar resources
P-47: Comparison of Sperm Acrosin Activity and DNA Fragmentation between Varicocele and Infertile Individuals
Background: Introduction assessment of sperm functional tests along with routine semen analysis may provide further information in the etiology of varicocele in different subpopulations. Therefore, in this study, in addition to assessment of sperm chromatin integrity, we aimed to assess the total acrosin activity which has proven to have major role during in vitro and natural fertilization in v...
full textP-59: Effect of Microsurgical Varicocelectomy on Sperm Chromatin Packaging,Miscarriage and Pregnancy Rate in Infertile Men with Varicocele
Background: Although the pathophysiology of the testicular damage associated with varicocele remains unclear, but varicocelectomy is considered a suitable technique for the treatment of individuals with clinical varicocele. The current study was designed to assessed semen parameters, chromatin packaging, and pregnancy outcome before and after varicocelectomy. Materials and Methods: Semen sample...
full textP-11: Evaluation of Heat Shock Protein A2 in Male Rats before and after Varicocele Induction
Background: Varicocele is a major cause of male infertility, as it may impair spermatogenesis through several distinct pathophysiological mechanisms including hypoxia, renal-adrenal reflux, hormonal dysfunction, autoimmunity, oxidative stress and hyperthermia. HSP70s are members of the heat shock protein (HSP) family, which are considered intracellular chaperones. Their expressions can increase...
full textP-4: Zaditen (Ketotifen), as Mast Cell Blocker, Improves Sperm Quality, Chromatin Integrity and Pregnancy Rate after Varicocelectomy
Background: Surgery is considered the most common choice for the treatment of male infertility with clinical varicocele. Increased numbers of mast cells (MCs) have been associated with different types of infertility, including varicocele. Despite there being different reports on improved fertility following administration of MC blockers, there is no report on the effect of a MC blocker on varic...
full textEtiology and Evaluation of Sperm Chromatin Anomalies
Evidence suggests that human sperm chromatin anomalies adversely affect reproductive outcomes and infertile men possess substantially amount of sperm with chromatin anomalies than fertile men. Routine semen analysis evaluates parameters such as sperm motility and morphology, but does not examine the nuclear DNA integrity of spermatozoa. It has been suggested that altered nuclear chromatin struc...
full textP-50: Evaluation of Sperm Parameters and Chromatin Abnormalities in Infertility with CASA and Chromatin Dispersion Test
Background: Although sperm selection according to morphologic and motility characteristic, made to some what the fertility successful, but sperm’s normal morphology cannot demonstrate sperm DNA competence. Since the health of sperm DNA is affecting the results of assisted reproductive technology; the aim of this study is to evaluate semen parameters measured by CASA with sperm chromatin abnorma...
full textMy Resources
Journal title
volume 5 issue Supplement Issue
pages -
publication date 2011-09-01
By following a journal you will be notified via email when a new issue of this journal is published.
Hosted on Doprax cloud platform doprax.com
copyright © 2015-2023